Regulatory
P-ldh

Part:BBa_K3183002:Design

Designed by: Natasha Cooke   Group: iGEM19_Oxford   (2019-09-20)


Lactate Dehydrogenase Constitutive Promoter for Lactobacillus sp.

The part was identified in the article by Lizier et al; the gene was codon optimised for L. reuteri and synthesized by IDT. The part was leter assembled by Gibson Assembly into the composite part BBa_K3183101, which was finally assembled into the pTRKH3 vector (BBa_K3183050), again by Gibson Assembly


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 219


Design Notes

This promoter was specifically selected as an ideal promoter for L. reuteri because it conformed to the RF10, RF12, RF21, RF23 and RF25 standards.

Source

The promoter is derived from the lactate dehydrogenase (ldlL) promoter from Lactobacillus acidophilus.

References

Lizier, Michela, et al. “Comparison of Expression Vectors in Lactobacillus Reuteri  Strains.” FEMS Microbiology Letters, vol. 308, no. 1, Aug. 2010, pp. 8–15., doi:10.1111/j.1574-6968.2010.01978.x.